Removal of Malachite Green by Using Immobilized Glucose Oxidase Onto Silica Nanostructure-Coated Silver Metal-Foam

Hemmati, Mansour; Barkhi, Mohammad; Baharifar, Hadi; Khoshnevisan, Kamyar

doi:10.22034/jna.2017.539362

|
|
|
How to cite
RIS EndNote BibTeX APA MLA Harvard Vancouver
|
Share

	Removal of Malachite Green by Using Immobilized Glucose Oxidase Onto Silica Nanostructure-Coated Silver Metal-Foam
Article 3, Volume 4, Issue 3, Autumn 2017, Page 196-204 PDF (948 K)
Document Type: Original Research Paper
DOI: 10.22034/jna.2017.539362
Authors
Mansour Hemmati ¹; Mohammad Barkhi²; Hadi Baharifar³; Kamyar Khoshnevisan ⁴
¹Nano Pooshesh Felez Co., University of Tehran science and Technology Park, Tehran, Iran
²University of Applied Science and Technology (UAST), Zar Branch, Karaj, Iran
³Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran
⁴Nano Pooshesh Felez Co., University of Tehran science and Technology Park, Tehran, Iran Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Enzymes Immobilization onto different types of the substrate could be helpful in various applications of biomedical devices and biosensors. Enzyme activity and stability could be affected by support and method of immobilization. In this study, the silver metal foam was successfully synthesized by the soft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on noncoated, and silica-coated silver metal foam and removal of malachite green was investigated. Fourier Transform Infrared Spectroscopy (FT-IR) and scanning electron microscopy (SEM) results confirmed that the enzyme was attached to support surface. The maximum immobilized enzyme activity was about 118.980 U/grSupport at 40°C. The removal of malachite green showed the indirect relation with dye concentration confirmed by decolorization assay. The high activity, thermal stability, and reusability of immobilized glucose oxidase onto the silver metal foam in comparison to free enzyme introduce the capability of the biological system for removal of malachite green in the industry.
Keywords
Enzyme immobilization; Glucose oxidase; Silver metal foam; Enzyme activity; Malachite green


Statistics Article View: 160 PDF Download: 46